;;; GNU Guix --- Functional package management for GNU ;;; Copyright © 2014, 2015 Ricardo Wurmus ;;; Copyright © 2015 Ben Woodcroft ;;; ;;; This file is part of GNU Guix. ;;; ;;; GNU Guix is free software; you can redistribute it and/or modify it ;;; under the terms of the GNU General Public License as published by ;;; the Free Software Foundation; either version 3 of the License, or (at ;;; your option) any later version. ;;; ;;; GNU Guix is distributed in the hope that it will be useful, but ;;; WITHOUT ANY WARRANTY; without even the implied warranty of ;;; MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the ;;; GNU General Public License for more details. ;;; ;;; You should have received a copy of the GNU General Public License ;;; along with GNU Guix. If not, see . (define-module (gnu packages bioinformatics) #:use-module ((guix licenses) #:prefix license:) #:use-module (guix packages) #:use-module (guix utils) #:use-module (guix download) #:use-module (guix git-download) #:use-module (guix build-system gnu) #:use-module (guix build-system cmake) #:use-module (guix build-system perl) #:use-module (guix build-system python) #:use-module (guix build-system ruby) #:use-module (guix build-system r) #:use-module (guix build-system trivial) #:use-module (gnu packages) #:use-module (gnu packages algebra) #:use-module (gnu packages base) #:use-module (gnu packages boost) #:use-module (gnu packages compression) #:use-module (gnu packages cpio) #:use-module (gnu packages file) #:use-module (gnu packages java) #:use-module (gnu packages linux) #:use-module (gnu packages machine-learning) #:use-module (gnu packages maths) #:use-module (gnu packages ncurses) #:use-module (gnu packages perl) #:use-module (gnu packages pkg-config) #:use-module (gnu packages popt) #:use-module (gnu packages protobuf) #:use-module (gnu packages python) #:use-module (gnu packages ruby) #:use-module (gnu packages statistics) #:use-module (gnu packages tbb) #:use-module (gnu packages textutils) #:use-module (gnu packages vim) #:use-module (gnu packages web) #:use-module (gnu packages xml) #:use-module (gnu packages zip) #:use-module (srfi srfi-1)) (define-public aragorn (package (name "aragorn") (version "1.2.36") (source (origin (method url-fetch) (uri (string-append "http://mbio-serv2.mbioekol.lu.se/ARAGORN/Downloads/aragorn" version ".tgz")) (sha256 (base32 "1dg7jlz1qpqy88igjxd6ncs11ccsirb36qv1z01a0np4i4jh61mb")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; there are no tests #:phases (modify-phases %standard-phases (delete 'configure) (replace 'build (lambda _ (zero? (system* "gcc" "-O3" "-ffast-math" "-finline-functions" "-o" "aragorn" (string-append "aragorn" ,version ".c"))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin")) (man (string-append out "/share/man/man1"))) (mkdir-p bin) (copy-file "aragorn" (string-append bin "/aragorn")) (mkdir-p man) (copy-file "aragorn.1" (string-append man "/aragorn.1"))) #t))))) (home-page "http://mbio-serv2.mbioekol.lu.se/ARAGORN") (synopsis "Detect tRNA, mtRNA and tmRNA genes in nucleotide sequences") (description "Aragorn identifies transfer RNA, mitochondrial RNA and transfer-messenger RNA from nucleotide sequences, based on homology to known tRNA consensus sequences and RNA structure. It also outputs the secondary structure of the predicted RNA.") (license license:gpl2))) (define-public bamtools (package (name "bamtools") (version "2.3.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/pezmaster31/bamtools/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1brry29bw2xr2l9pqn240rkqwayg85b8qq78zk2zs6nlspk4d018")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-before 'configure 'set-ldflags (lambda* (#:key outputs #:allow-other-keys) (setenv "LDFLAGS" (string-append "-Wl,-rpath=" (assoc-ref outputs "out") "/lib/bamtools"))))))) (inputs `(("zlib" ,zlib))) (home-page "https://github.com/pezmaster31/bamtools") (synopsis "C++ API and command-line toolkit for working with BAM data") (description "BamTools provides both a C++ API and a command-line toolkit for handling BAM files.") (license license:expat))) (define-public bedops (package (name "bedops") (version "2.4.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/bedops/bedops/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1kqbac547wyqma81cyky9n7mkgikjpsfd3nnmcm6hpqwanqgh10v")))) (build-system gnu-build-system) (arguments '(#:tests? #f #:make-flags (list (string-append "BINDIR=" %output "/bin")) #:phases (alist-cons-after 'unpack 'unpack-tarballs (lambda _ ;; FIXME: Bedops includes tarballs of minimally patched upstream ;; libraries jansson, zlib, and bzip2. We cannot just use stock ;; libraries because at least one of the libraries (zlib) is ;; patched to add a C++ function definition (deflateInit2cpp). ;; Until the Bedops developers offer a way to link against system ;; libraries we have to build the in-tree copies of these three ;; libraries. ;; See upstream discussion: ;; https://github.com/bedops/bedops/issues/124 ;; Unpack the tarballs to benefit from shebang patching. (with-directory-excursion "third-party" (and (zero? (system* "tar" "xvf" "jansson-2.6.tar.bz2")) (zero? (system* "tar" "xvf" "zlib-1.2.7.tar.bz2")) (zero? (system* "tar" "xvf" "bzip2-1.0.6.tar.bz2")))) ;; Disable unpacking of tarballs in Makefile. (substitute* "system.mk/Makefile.linux" (("^\tbzcat .*") "\t@echo \"not unpacking\"\n") (("\\./configure") "CONFIG_SHELL=bash ./configure")) (substitute* "third-party/zlib-1.2.7/Makefile.in" (("^SHELL=.*$") "SHELL=bash\n"))) (alist-delete 'configure %standard-phases)))) (home-page "https://github.com/bedops/bedops") (synopsis "Tools for high-performance genomic feature operations") (description "BEDOPS is a suite of tools to address common questions raised in genomic studies---mostly with regard to overlap and proximity relationships between data sets. It aims to be scalable and flexible, facilitating the efficient and accurate analysis and management of large-scale genomic data. BEDOPS provides tools that perform highly efficient and scalable Boolean and other set operations, statistical calculations, archiving, conversion and other management of genomic data of arbitrary scale. Tasks can be easily split by chromosome for distributing whole-genome analyses across a computational cluster.") (license license:gpl2+))) (define-public bedtools (package (name "bedtools") (version "2.24.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/arq5x/bedtools2/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0lnxrjvs3nnmb4bmskag1wg3h2hd80przz5q3xd0bvs7vyxrvpbl")) (patches (list (search-patch "bedtools-32bit-compilation.patch"))))) (build-system gnu-build-system) (native-inputs `(("python" ,python-2))) (inputs `(("samtools" ,samtools) ("zlib" ,zlib))) (arguments '(#:test-target "test" #:phases (alist-cons-after 'unpack 'patch-makefile-SHELL-definition (lambda _ ;; patch-makefile-SHELL cannot be used here as it does not ;; yet patch definitions with `:='. Since changes to ;; patch-makefile-SHELL result in a full rebuild, features ;; of patch-makefile-SHELL are reimplemented here. (substitute* "Makefile" (("^SHELL := .*$") (string-append "SHELL := " (which "bash") " -e \n")))) (alist-delete 'configure (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (for-each (lambda (file) (copy-file file (string-append bin (basename file)))) (find-files "bin" ".*")))) %standard-phases))))) (home-page "https://github.com/arq5x/bedtools2") (synopsis "Tools for genome analysis and arithmetic") (description "Collectively, the bedtools utilities are a swiss-army knife of tools for a wide-range of genomics analysis tasks. The most widely-used tools enable genome arithmetic: that is, set theory on the genome. For example, bedtools allows one to intersect, merge, count, complement, and shuffle genomic intervals from multiple files in widely-used genomic file formats such as BAM, BED, GFF/GTF, VCF.") (license license:gpl2))) (define-public python2-pybedtools (package (name "python2-pybedtools") (version "0.6.9") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/p/pybedtools/pybedtools-" version ".tar.gz")) (sha256 (base32 "1ldzdxw1p4y3g2ignmggsdypvqkcwqwzhdha4rbgpih048z5p4an")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; no Python 3 support (inputs `(("python-cython" ,python2-cython) ("python-matplotlib" ,python2-matplotlib))) (propagated-inputs `(("bedtools" ,bedtools) ("samtools" ,samtools))) (native-inputs `(("python-pyyaml" ,python2-pyyaml) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "https://pythonhosted.org/pybedtools/") (synopsis "Python wrapper for BEDtools programs") (description "pybedtools is a Python wrapper for Aaron Quinlan's BEDtools programs, which are widely used for genomic interval manipulation or \"genome algebra\". pybedtools extends BEDTools by offering feature-level manipulations from with Python.") (license license:gpl2+))) (define-public bioperl-minimal (let* ((inputs `(("perl-module-build" ,perl-module-build) ("perl-data-stag" ,perl-data-stag) ("perl-libwww" ,perl-libwww) ("perl-uri" ,perl-uri))) (transitive-inputs (map (compose package-name cadr) (delete-duplicates (concatenate (map (compose package-transitive-target-inputs cadr) inputs)))))) (package (name "bioperl-minimal") (version "1.6.924") (source (origin (method url-fetch) (uri (string-append "mirror://cpan/authors/id/C/CJ/CJFIELDS/BioPerl-" version ".tar.gz")) (sha256 (base32 "1l3npcvvvwjlhkna9dndpfv1hklhrgva013kw96m0n1wpd37ask1")))) (build-system perl-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-programs (lambda* (#:key outputs #:allow-other-keys) ;; Make sure all executables in "bin" find the required Perl ;; modules at runtime. As the PERL5LIB variable contains also ;; the paths of native inputs, we pick the transitive target ;; inputs from %build-inputs. (let* ((out (assoc-ref outputs "out")) (bin (string-append out "/bin/")) (path (string-join (cons (string-append out "/lib/perl5/site_perl") (map (lambda (name) (assoc-ref %build-inputs name)) ',transitive-inputs)) ":"))) (for-each (lambda (file) (wrap-program file `("PERL5LIB" ":" prefix (,path)))) (find-files bin "\\.pl$")) #t)))))) (inputs inputs) (native-inputs `(("perl-test-most" ,perl-test-most))) (home-page "http://search.cpan.org/dist/BioPerl") (synopsis "Bioinformatics toolkit") (description "BioPerl is the product of a community effort to produce Perl code which is useful in biology. Examples include Sequence objects, Alignment objects and database searching objects. These objects not only do what they are advertised to do in the documentation, but they also interact - Alignment objects are made from the Sequence objects, Sequence objects have access to Annotation and SeqFeature objects and databases, Blast objects can be converted to Alignment objects, and so on. This means that the objects provide a coordinated and extensible framework to do computational biology.") (license (package-license perl))))) (define-public python-biopython (package (name "python-biopython") (version "1.65") (source (origin (method url-fetch) (uri (string-append "http://biopython.org/DIST/biopython-" version ".tar.gz")) (sha256 (base32 "13m8s9jkrw40zvdp1rl709n6lmgdh4f52aann7gzr6sfp0fwhg26")))) (build-system python-build-system) (inputs `(("python-numpy" ,python-numpy))) (native-inputs `(("python-setuptools" ,python2-setuptools))) (home-page "http://biopython.org/") (synopsis "Tools for biological computation in Python") (description "Biopython is a set of tools for biological computation including parsers for bioinformatics files into Python data structures; interfaces to common bioinformatics programs; a standard sequence class and tools for performing common operations on them; code to perform data classification; code for dealing with alignments; code making it easy to split up parallelizable tasks into separate processes; and more.") (license (license:non-copyleft "http://www.biopython.org/DIST/LICENSE")))) (define-public python2-biopython (package (inherit (package-with-python2 python-biopython)) (inputs `(("python2-numpy" ,python2-numpy))))) (define-public blast+ (package (name "blast+") (version "2.2.31") (source (origin (method url-fetch) (uri (string-append "ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/" version "/ncbi-blast-" version "+-src.tar.gz")) (sha256 (base32 "19gq6as4k1jrgsd26158ads6h7v4jca3h4r5dzg1y0m6ya50x5ph")) (modules '((guix build utils))) (snippet '(begin ;; Remove bundled bzip2 and zlib (delete-file-recursively "c++/src/util/compress/bzip2") (delete-file-recursively "c++/src/util/compress/zlib") (substitute* "c++/src/util/compress/Makefile.in" (("bzip2 zlib api") "api")) ;; Remove useless msbuild directory (delete-file-recursively "c++/src/build-system/project_tree_builder/msbuild") #t)))) (build-system gnu-build-system) (arguments `(;; There are three(!) tests for this massive library, and all fail with ;; "unparsable timing stats". ;; ERR [127] -- [util/regexp] test_pcre.sh (unparsable timing stats) ;; ERR [127] -- [serial/datatool] datatool.sh (unparsable timing stats) ;; ERR [127] -- [serial/datatool] datatool_xml.sh (unparsable timing stats) #:tests? #f #:out-of-source? #t #:parallel-build? #f ; not supported #:phases (modify-phases %standard-phases (add-before 'configure 'set-HOME ;; $HOME needs to be set at some point during the configure phase (lambda _ (setenv "HOME" "/tmp") #t)) (add-after 'unpack 'enter-dir (lambda _ (chdir "c++") #t)) (add-after 'enter-dir 'fix-build-system (lambda _ (define (which* cmd) (cond ((string=? cmd "date") ;; make call to "date" deterministic "date -d @0") ((which cmd) => identity) (else (format (current-error-port) "WARNING: Unable to find absolute path for ~s~%" cmd) #f))) ;; Rewrite hardcoded paths to various tools (substitute* (append '("src/build-system/configure.ac" "src/build-system/configure" "scripts/common/impl/if_diff.sh" "scripts/common/impl/run_with_lock.sh" "src/build-system/Makefile.configurables.real" "src/build-system/Makefile.in.top" "src/build-system/Makefile.meta.gmake=no" "src/build-system/Makefile.meta.in" "src/build-system/Makefile.meta_l" "src/build-system/Makefile.meta_p" "src/build-system/Makefile.meta_r" "src/build-system/Makefile.mk.in" "src/build-system/Makefile.requirements" "src/build-system/Makefile.rules_with_autodep.in") (find-files "scripts/common/check" "\\.sh$")) (("(/usr/bin/|/bin/)([a-z][-_.a-z]*)" all dir cmd) (or (which* cmd) all))) (substitute* (find-files "src/build-system" "^config.*") (("LN_S=/bin/\\$LN_S") (string-append "LN_S=" (which "ln"))) (("^PATH=.*") "")) ;; rewrite "/var/tmp" in check script (substitute* "scripts/common/check/check_make_unix.sh" (("/var/tmp") "/tmp")) ;; do not reset PATH (substitute* (find-files "scripts/common/impl/" "\\.sh$") (("^ *PATH=.*") "") (("action=/bin/") "action=") (("export PATH") ":")) #t)) (replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out")) (lib (string-append (assoc-ref outputs "lib") "/lib")) (include (string-append (assoc-ref outputs "include") "/include/ncbi-tools++"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure.orig" (string-append "--with-build-root=" (getcwd) "/build") (string-append "--prefix=" out) (string-append "--libdir=" lib) (string-append "--includedir=" include) (string-append "--with-bz2=" (assoc-ref inputs "bzip2")) (string-append "--with-z=" (assoc-ref inputs "zlib")) ;; Each library is built twice by default, once ;; with "-static" in its name, and again ;; without. "--without-static" "--with-dll")))))))) (outputs '("out" ; 19 MB "lib" ; 203 MB "include")) ; 32 MB (inputs `(("bzip2" ,bzip2) ("zlib" ,zlib))) (native-inputs `(("cpio" ,cpio))) (home-page "http://blast.ncbi.nlm.nih.gov") (synopsis "Basic local alignment search tool") (description "BLAST is a popular method of performing a DNA or protein sequence similarity search, using heuristics to produce results quickly. It also calculates an “expect value” that estimates how many matches would have occurred at a given score by chance, which can aid a user in judging how much confidence to have in an alignment.") ;; Most of the sources are in the public domain, with the following ;; exceptions: ;; * Expat: ;; * ./c++/include/util/bitset/ ;; * ./c++/src/html/ncbi_menu*.js ;; * Boost license: ;; * ./c++/include/util/impl/floating_point_comparison.hpp ;; * LGPL 2+: ;; * ./c++/include/dbapi/driver/odbc/unix_odbc/ ;; * ASL 2.0: ;; * ./c++/src/corelib/teamcity_* (license (list license:public-domain license:expat license:boost1.0 license:lgpl2.0+ license:asl2.0)))) (define-public bowtie (package (name "bowtie") (version "2.2.4") (source (origin (method url-fetch) (uri (string-append "https://github.com/BenLangmead/bowtie2/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "15dnbqippwvhyh9zqjhaxkabk7lm1xbh1nvar1x4b5kwm117zijn")) (modules '((guix build utils))) (snippet '(substitute* "Makefile" (("^CC = .*$") "CC = gcc") (("^CPP = .*$") "CPP = g++") ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\""))) (patches (list (search-patch "bowtie-fix-makefile.patch"))))) (build-system gnu-build-system) (inputs `(("perl" ,perl) ("perl-clone" ,perl-clone) ("perl-test-deep" ,perl-test-deep) ("perl-test-simple" ,perl-test-simple) ("python" ,python-2))) (arguments '(#:make-flags '("allall") #:phases (alist-delete 'configure (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (for-each (lambda (file) (copy-file file (string-append bin file))) (find-files "." "bowtie2.*")))) (alist-replace 'check (lambda* (#:key outputs #:allow-other-keys) (system* "perl" "scripts/test/simple_tests.pl" "--bowtie2=./bowtie2" "--bowtie2-build=./bowtie2-build")) %standard-phases))))) (home-page "http://bowtie-bio.sourceforge.net/bowtie2/index.shtml") (synopsis "Fast and sensitive nucleotide sequence read aligner") (description "Bowtie 2 is a fast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB. Bowtie 2 supports gapped, local, and paired-end alignment modes.") (supported-systems '("x86_64-linux")) (license license:gpl3+))) (define-public bwa (package (name "bwa") (version "0.7.12") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/bio-bwa/bwa-" version ".tar.bz2")) (sha256 (base32 "1330dpqncv0px3pbhjzz1gwgg39kkcv2r9qp2xs0sixf8z8wl7bh")))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:phases (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin")) (doc (string-append (assoc-ref outputs "out") "/share/doc/bwa")) (man (string-append (assoc-ref outputs "out") "/share/man/man1"))) (mkdir-p bin) (mkdir-p doc) (mkdir-p man) (copy-file "bwa" (string-append bin "/bwa")) (copy-file "README.md" (string-append doc "/README.md")) (copy-file "bwa.1" (string-append man "/bwa.1")))) ;; no "configure" script (alist-delete 'configure %standard-phases)))) (inputs `(("zlib" ,zlib))) (home-page "http://bio-bwa.sourceforge.net/") (synopsis "Burrows-Wheeler sequence aligner") (description "BWA is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome. It consists of three algorithms: BWA-backtrack, BWA-SW and BWA-MEM. The first algorithm is designed for Illumina sequence reads up to 100bp, while the rest two for longer sequences ranged from 70bp to 1Mbp. BWA-MEM and BWA-SW share similar features such as long-read support and split alignment, but BWA-MEM, which is the latest, is generally recommended for high-quality queries as it is faster and more accurate. BWA-MEM also has better performance than BWA-backtrack for 70-100bp Illumina reads.") (license license:gpl3+))) (define-public python2-bx-python (package (name "python2-bx-python") (version "0.7.2") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/b/bx-python/bx-python-" version ".tar.gz")) (sha256 (base32 "0ld49idhc5zjdvbhvjq1a2qmpjj7h5v58rqr25dzmfq7g34b50xh")) (modules '((guix build utils))) (snippet '(substitute* "setup.py" ;; remove dependency on outdated "distribute" module (("^from distribute_setup import use_setuptools") "") (("^use_setuptools\\(\\)") ""))))) (build-system python-build-system) (arguments `(#:tests? #f ;tests fail because test data are not included #:python ,python-2)) (inputs `(("python-numpy" ,python2-numpy) ("zlib" ,zlib))) (native-inputs `(("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "http://bitbucket.org/james_taylor/bx-python/") (synopsis "Tools for manipulating biological data") (description "bx-python provides tools for manipulating biological data, particularly multiple sequence alignments.") (license license:expat))) (define-public clipper (package (name "clipper") (version "0.3.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/YeoLab/clipper/archive/" version ".tar.gz")) (sha256 (base32 "1q7jpimsqln7ic44i8v2rx2haj5wvik8hc1s2syd31zcn0xk1iyq")) (modules '((guix build utils))) (snippet ;; remove unnecessary setup dependency '(substitute* "setup.py" (("setup_requires = .*") ""))))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; only Python 2 is supported (inputs `(("htseq" ,htseq) ("python-pybedtools" ,python2-pybedtools) ("python-cython" ,python2-cython) ("python-scikit-learn" ,python2-scikit-learn) ("python-matplotlib" ,python2-matplotlib) ("python-pysam" ,python2-pysam) ("python-numpy" ,python2-numpy) ("python-scipy" ,python2-scipy))) (native-inputs `(("python-mock" ,python2-mock) ; for tests ("python-pytz" ,python2-pytz) ; for tests ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/YeoLab/clipper") (synopsis "CLIP peak enrichment recognition") (description "CLIPper is a tool to define peaks in CLIP-seq datasets.") (license license:gpl2))) (define-public couger (package (name "couger") (version "1.8.2") (source (origin (method url-fetch) (uri (string-append "http://couger.oit.duke.edu/static/assets/COUGER" version ".zip")) (sha256 (base32 "04p2b14nmhzxw5h72mpzdhalv21bx4w9b87z0wpw0xzxpysyncmq")))) (build-system gnu-build-system) (arguments `(#:tests? #f #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (copy-recursively "src" (string-append out "/src")) (mkdir (string-append out "/bin")) ;; Add "src" directory to module lookup path. (substitute* "couger" (("from argparse") (string-append "import sys\nsys.path.append(\"" out "\")\nfrom argparse"))) (copy-file "couger" (string-append out "/bin/couger"))) #t)) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) ;; Make sure 'couger' runs with the correct PYTHONPATH. (let* ((out (assoc-ref outputs "out")) (path (getenv "PYTHONPATH"))) (wrap-program (string-append out "/bin/couger") `("PYTHONPATH" ":" prefix (,path)))) #t))))) (inputs `(("python" ,python-2) ("python2-pillow" ,python2-pillow) ("python2-numpy" ,python2-numpy) ("python2-scipy" ,python2-scipy) ("python2-matplotlib" ,python2-matplotlib))) (propagated-inputs `(("r" ,r) ("libsvm" ,libsvm) ("randomjungle" ,randomjungle))) (native-inputs `(("unzip" ,unzip))) (home-page "http://couger.oit.duke.edu") (synopsis "Identify co-factors in sets of genomic regions") (description "COUGER can be applied to any two sets of genomic regions bound by paralogous TFs (e.g., regions derived from ChIP-seq experiments) to identify putative co-factors that provide specificity to each TF. The framework determines the genomic targets uniquely-bound by each TF, and identifies a small set of co-factors that best explain the in vivo binding differences between the two TFs. COUGER uses classification algorithms (support vector machines and random forests) with features that reflect the DNA binding specificities of putative co-factors. The features are generated either from high-throughput TF-DNA binding data (from protein binding microarray experiments), or from large collections of DNA motifs.") (license license:gpl3+))) (define-public clustal-omega (package (name "clustal-omega") (version "1.2.1") (source (origin (method url-fetch) (uri (string-append "http://www.clustal.org/omega/clustal-omega-" version ".tar.gz")) (sha256 (base32 "02ibkx0m0iwz8nscg998bh41gg251y56cgh86bvyrii5m8kjgwqf")))) (build-system gnu-build-system) (inputs `(("argtable" ,argtable))) (home-page "http://www.clustal.org/omega/") (synopsis "Multiple sequence aligner for protein and DNA/RNA") (description "Clustal-Omega is a general purpose multiple sequence alignment (MSA) program for protein and DNA/RNA. It produces high quality MSAs and is capable of handling data-sets of hundreds of thousands of sequences in reasonable time.") (license license:gpl2+))) (define-public crossmap (package (name "crossmap") (version "0.1.6") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/crossmap/CrossMap-" version ".tar.gz")) (sha256 (base32 "163hi5gjgij6cndxlvbkp5jjwr0k4wbm9im6d2210278q7k9kpnp")) ;; patch has been sent upstream already (patches (list (search-patch "crossmap-allow-system-pysam.patch"))) (modules '((guix build utils))) ;; remove bundled copy of pysam (snippet '(delete-file-recursively "lib/pysam")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (alist-cons-after 'unpack 'set-env (lambda _ (setenv "CROSSMAP_USE_SYSTEM_PYSAM" "1")) %standard-phases))) (inputs `(("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("zlib" ,zlib))) (native-inputs `(("python-cython" ,python2-cython) ("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "http://crossmap.sourceforge.net/") (synopsis "Convert genome coordinates between assemblies") (description "CrossMap is a program for conversion of genome coordinates or annotation files between different genome assemblies. It supports most commonly used file formats including SAM/BAM, Wiggle/BigWig, BED, GFF/GTF, VCF.") (license license:gpl2+))) (define-public cutadapt (package (name "cutadapt") (version "1.8") (source (origin (method url-fetch) (uri (string-append "https://github.com/marcelm/cutadapt/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "161bp87y6gd6r5bmvjpn2b1k942i3fizfpa139f0jn6jv1wcp5h5")))) (build-system python-build-system) (arguments ;; tests must be run after install `(#:phases (alist-cons-after 'install 'check (lambda* (#:key inputs outputs #:allow-other-keys) (setenv "PYTHONPATH" (string-append (getenv "PYTHONPATH") ":" (assoc-ref outputs "out") "/lib/python" (string-take (string-take-right (assoc-ref inputs "python") 5) 3) "/site-packages")) (zero? (system* "nosetests" "-P" "tests"))) (alist-delete 'check %standard-phases)))) (native-inputs `(("python-cython" ,python-cython) ("python-nose" ,python-nose) ("python-setuptools" ,python-setuptools))) (home-page "https://code.google.com/p/cutadapt/") (synopsis "Remove adapter sequences from nucleotide sequencing reads") (description "Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from high-throughput sequencing reads.") (license license:expat))) (define-public deeptools (package (name "deeptools") (version "1.5.11") (source (origin (method url-fetch) (uri (string-append "https://github.com/fidelram/deepTools/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1kaagygcbvjs9sxd9cqmskd02wcfp9imvb735r087w7hwqpvz6fs")))) (build-system python-build-system) (arguments `(#:python ,python-2)) (propagated-inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-matplotlib" ,python2-matplotlib) ("python-bx-python" ,python2-bx-python) ("python-pysam" ,python2-pysam))) (native-inputs `(("python-mock" ,python2-mock) ;for tests ("python-pytz" ,python2-pytz) ;for tests ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/fidelram/deepTools") (synopsis "Tools for normalizing and visualizing deep-sequencing data") (description "DeepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. To do so, deepTools contains useful modules to process the mapped reads data to create coverage files in standard bedGraph and bigWig file formats. By doing so, deepTools allows the creation of normalized coverage files or the comparison between two files (for example, treatment and control). Finally, using such normalized and standardized files, multiple visualizations can be created to identify enrichments with functional annotations of the genome.") (license license:gpl3+))) (define-public diamond (package (name "diamond") (version "0.7.9") (source (origin (method url-fetch) (uri (string-append "https://github.com/bbuchfink/diamond/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0hfkcfv9f76h5brbyw9fyvmc0l9cmbsxrcdqk0fa9xv82zj47p15")) (snippet '(begin (delete-file "bin/diamond") #t)))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-source-dir (lambda _ (chdir "src") #t)) (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "../bin/diamond" (string-append bin "/diamond")) #t)))))) (native-inputs `(("bc" ,bc))) (inputs `(("boost" ,boost) ("zlib" ,zlib))) (home-page "https://github.com/bbuchfink/diamond") (synopsis "Accelerated BLAST compatible local sequence aligner") (description "DIAMOND is a BLAST-compatible local aligner for mapping protein and translated DNA query sequences against a protein reference database (BLASTP and BLASTX alignment mode). The speedup over BLAST is up to 20,000 on short reads at a typical sensitivity of 90-99% relative to BLAST depending on the data and settings.") (license (license:non-copyleft "file://src/COPYING" "See src/COPYING in the distribution.")))) (define-public edirect (package (name "edirect") (version "2.50") (source (origin (method url-fetch) ;; Note: older versions are not retained. (uri "ftp://ftp.ncbi.nlm.nih.gov/entrez/entrezdirect/edirect.zip") (sha256 (base32 "08afhz2ph66h8h381hl1mqyxkdi5nbvzsyj9gfw3jfbdijnpi4qj")))) (build-system perl-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (delete 'configure) (delete 'build) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((target (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p target) (copy-file "edirect.pl" (string-append target "/edirect.pl")) #t))) (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) ;; Make sure 'edirect.pl' finds all perl inputs at runtime. (let* ((out (assoc-ref outputs "out")) (path (getenv "PERL5LIB"))) (wrap-program (string-append out "/bin/edirect.pl") `("PERL5LIB" ":" prefix (,path))))))))) (inputs `(("perl-html-parser" ,perl-html-parser) ("perl-encode-locale" ,perl-encode-locale) ("perl-file-listing" ,perl-file-listing) ("perl-html-tagset" ,perl-html-tagset) ("perl-html-tree" ,perl-html-tree) ("perl-http-cookies" ,perl-http-cookies) ("perl-http-date" ,perl-http-date) ("perl-http-message" ,perl-http-message) ("perl-http-negotiate" ,perl-http-negotiate) ("perl-lwp-mediatypes" ,perl-lwp-mediatypes) ("perl-lwp-protocol-https" ,perl-lwp-protocol-https) ("perl-net-http" ,perl-net-http) ("perl-uri" ,perl-uri) ("perl-www-robotrules" ,perl-www-robotrules) ("perl" ,perl))) (native-inputs `(("unzip" ,unzip))) (home-page "http://www.ncbi.nlm.nih.gov/books/NBK179288") (synopsis "Tools for accessing the NCBI's set of databases") (description "Entrez Direct (EDirect) is a method for accessing the National Center for Biotechnology Information's (NCBI) set of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a terminal. Functions take search terms from command-line arguments. Individual operations are combined to build multi-step queries. Record retrieval and formatting normally complete the process. EDirect also provides an argument-driven function that simplifies the extraction of data from document summaries or other results that are returned in structured XML format. This can eliminate the need for writing custom software to answer ad hoc questions.") (license license:public-domain))) (define-public express (package (name "express") (version "1.5.1") (source (origin (method url-fetch) (uri (string-append "http://bio.math.berkeley.edu/eXpress/downloads/express-" version "/express-" version "-src.tgz")) (sha256 (base32 "03rczxd0gjp2l1jxcmjfmf5j94j77zqyxa6x063zsc585nj40n0c")))) (build-system cmake-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (alist-cons-after 'unpack 'use-shared-boost-libs-and-set-bamtools-paths (lambda* (#:key inputs #:allow-other-keys) (substitute* "CMakeLists.txt" (("set\\(Boost_USE_STATIC_LIBS ON\\)") "set(Boost_USE_STATIC_LIBS OFF)") (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/bamtools/include") (string-append (assoc-ref inputs "bamtools") "/include/bamtools"))) (substitute* "src/CMakeLists.txt" (("\\$\\{CMAKE_CURRENT_SOURCE_DIR\\}/\\.\\./bamtools/lib") (string-append (assoc-ref inputs "bamtools") "/lib/bamtools"))) #t) %standard-phases))) (inputs `(("boost" ,boost) ("bamtools" ,bamtools) ("protobuf" ,protobuf) ("zlib" ,zlib))) (home-page "http://bio.math.berkeley.edu/eXpress") (synopsis "Streaming quantification for high-throughput genomic sequencing") (description "eXpress is a streaming tool for quantifying the abundances of a set of target sequences from sampled subsequences. Example applications include transcript-level RNA-Seq quantification, allele-specific/haplotype expression analysis (from RNA-Seq), transcription factor binding quantification in ChIP-Seq, and analysis of metagenomic data.") (license license:artistic2.0))) (define-public fasttree (package (name "fasttree") (version "2.1.8") (source (origin (method url-fetch) (uri (string-append "http://www.microbesonline.org/fasttree/FastTree-" version ".c")) (sha256 (base32 "0dzqc9vr9iiiw21y159xfjl2z90vw0y7r4x6456pcaxiy5hd2wmi")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:phases (modify-phases %standard-phases (delete 'unpack) (delete 'configure) (replace 'build (lambda* (#:key source #:allow-other-keys) (and (zero? (system* "gcc" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTree" source "-lm")) (zero? (system* "gcc" "-DOPENMP" "-fopenmp" "-O3" "-finline-functions" "-funroll-loops" "-Wall" "-o" "FastTreeMP" source "-lm"))))) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "FastTree" (string-append bin "/FastTree")) (copy-file "FastTreeMP" (string-append bin "/FastTreeMP")) #t)))))) (home-page "http://www.microbesonline.org/fasttree") (synopsis "Infers approximately-maximum-likelihood phylogenetic trees") (description "FastTree can handle alignments with up to a million of sequences in a reasonable amount of time and memory. For large alignments, FastTree is 100-1,000 times faster than PhyML 3.0 or RAxML 7.") (license license:gpl2+))) (define-public fastx-toolkit (package (name "fastx-toolkit") (version "0.0.14") (source (origin (method url-fetch) (uri (string-append "https://github.com/agordon/fastx_toolkit/releases/download/" version "/fastx_toolkit-" version ".tar.bz2")) (sha256 (base32 "01jqzw386873sr0pjp1wr4rn8fsga2vxs1qfmicvx1pjr72007wy")))) (build-system gnu-build-system) (inputs `(("libgtextutils" ,libgtextutils))) (native-inputs `(("pkg-config" ,pkg-config))) (home-page "http://hannonlab.cshl.edu/fastx_toolkit/") (synopsis "Tools for FASTA/FASTQ file preprocessing") (description "The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing. Next-Generation sequencing machines usually produce FASTA or FASTQ files, containing multiple short-reads sequences. The main processing of such FASTA/FASTQ files is mapping the sequences to reference genomes. However, it is sometimes more productive to preprocess the files before mapping the sequences to the genome---manipulating the sequences to produce better mapping results. The FASTX-Toolkit tools perform some of these preprocessing tasks.") (license license:agpl3+))) (define-public flexbar (package (name "flexbar") (version "2.5") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/flexbar/" version "/flexbar_v" version "_src.tgz")) (sha256 (base32 "13jaykc3y1x8y5nn9j8ljnb79s5y51kyxz46hdmvvjj6qhyympmf")))) (build-system cmake-build-system) (arguments `(#:configure-flags (list (string-append "-DFLEXBAR_BINARY_DIR=" (assoc-ref %outputs "out") "/bin/")) #:phases (alist-replace 'check (lambda* (#:key outputs #:allow-other-keys) (setenv "PATH" (string-append (assoc-ref outputs "out") "/bin:" (getenv "PATH"))) (chdir "../flexbar_v2.5_src/test") (zero? (system* "bash" "flexbar_validate.sh"))) (alist-delete 'install %standard-phases)))) (inputs `(("tbb" ,tbb) ("zlib" ,zlib))) (native-inputs `(("pkg-config" ,pkg-config) ("seqan" ,seqan))) (home-page "http://flexbar.sourceforge.net") (synopsis "Barcode and adapter removal tool for sequencing platforms") (description "Flexbar preprocesses high-throughput nucleotide sequencing data efficiently. It demultiplexes barcoded runs and removes adapter sequences. Moreover, trimming and filtering features are provided. Flexbar increases read mapping rates and improves genome and transcriptome assemblies. It supports next-generation sequencing data in fasta/q and csfasta/q format from Illumina, Roche 454, and the SOLiD platform.") (license license:gpl3))) (define-public grit (package (name "grit") (version "2.0.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/nboley/grit/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "157in84dj70wimbind3x7sy1whs3h57qfgcnj2s6lrd38fbrb7mj")))) (build-system python-build-system) (arguments `(#:python ,python-2 #:phases (alist-cons-after 'unpack 'generate-from-cython-sources (lambda* (#:key inputs outputs #:allow-other-keys) ;; Delete these C files to force fresh generation from pyx sources. (delete-file "grit/sparsify_support_fns.c") (delete-file "grit/call_peaks_support_fns.c") (substitute* "setup.py" (("Cython.Setup") "Cython.Build") ;; Add numpy include path to fix compilation (("pyx\", \\]") (string-append "pyx\", ], include_dirs = ['" (assoc-ref inputs "python-numpy") "/lib/python2.7/site-packages/numpy/core/include/" "']"))) #t) %standard-phases))) (inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-networkx" ,python2-networkx))) (native-inputs `(("python-cython" ,python2-cython) ("python-setuptools" ,python2-setuptools))) (home-page "http://grit-bio.org") (synopsis "Tool for integrative analysis of RNA-seq type assays") (description "GRIT is designed to use RNA-seq, TES, and TSS data to build and quantify full length transcript models. When none of these data sources are available, GRIT can be run by providing a candidate set of TES or TSS sites. In addition, GRIT can merge in reference junctions and gene boundaries. GRIT can also be run in quantification mode, where it uses a provided GTF file and just estimates transcript expression.") (license license:gpl3+))) (define-public hisat (package (name "hisat") (version "0.1.4") (source (origin (method url-fetch) (uri (string-append "http://ccb.jhu.edu/software/hisat/downloads/hisat-" version "-beta-source.zip")) (sha256 (base32 "1k381ydranqxp09yf2y7w1d0chz5d59vb6jchi89hbb0prq19lk5")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no check target #:make-flags '("allall" ;; Disable unsupported `popcnt' instructions on ;; architectures other than x86_64 ,@(if (string-prefix? "x86_64" (or (%current-target-system) (%current-system))) '() '("POPCNT_CAPABILITY=0"))) #:phases (alist-cons-after 'unpack 'patch-sources (lambda _ ;; XXX Cannot use snippet because zip files are not supported (substitute* "Makefile" (("^CC = .*$") "CC = gcc") (("^CPP = .*$") "CPP = g++") ;; replace BUILD_HOST and BUILD_TIME for deterministic build (("-DBUILD_HOST=.*") "-DBUILD_HOST=\"\\\"guix\\\"\"") (("-DBUILD_TIME=.*") "-DBUILD_TIME=\"\\\"0\\\"\"")) (substitute* '("hisat-build" "hisat-inspect") (("/usr/bin/env") (which "env")))) (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (for-each (lambda (file) (copy-file file (string-append bin file))) (find-files "." "hisat(-(build|align|inspect)(-(s|l)(-debug)*)*)*$")))) (alist-delete 'configure %standard-phases))))) (native-inputs `(("unzip" ,unzip))) (inputs `(("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) (home-page "http://ccb.jhu.edu/software/hisat/index.shtml") (synopsis "Hierarchical indexing for spliced alignment of transcripts") (description "HISAT is a fast and sensitive spliced alignment program for mapping RNA-seq reads. In addition to one global FM index that represents a whole genome, HISAT uses a large set of small FM indexes that collectively cover the whole genome. These small indexes (called local indexes) combined with several alignment strategies enable effective alignment of RNA-seq reads, in particular, reads spanning multiple exons.") (license license:gpl3+))) (define-public hmmer (package (name "hmmer") (version "3.1b2") (source (origin (method url-fetch) (uri (string-append "http://selab.janelia.org/software/hmmer" (version-prefix version 1) "/" version "/hmmer-" version ".tar.gz")) (sha256 (base32 "0djmgc0pfli0jilfx8hql1axhwhqxqb8rxg2r5rg07aw73sfs5nx")))) (build-system gnu-build-system) (native-inputs `(("perl", perl))) (home-page "http://hmmer.janelia.org") (synopsis "Biosequence analysis using profile hidden Markov models") (description "HMMER is used for searching sequence databases for homologs of protein sequences, and for making protein sequence alignments. It implements methods using probabilistic models called profile hidden Markov models (profile HMMs).") (license (list license:gpl3+ ;; The bundled library 'easel' is distributed ;; under The Janelia Farm Software License. (license:non-copyleft "file://easel/LICENSE" "See easel/LICENSE in the distribution."))))) (define-public htseq (package (name "htseq") (version "0.6.1") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/H/HTSeq/HTSeq-" version ".tar.gz")) (sha256 (base32 "1i85ppf2j2lj12m0x690qq5nn17xxk23pbbx2c83r8ayb5wngzwv")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; only Python 2 is supported (inputs `(("python-numpy" ,python2-numpy) ("python-setuptools" ,python2-setuptools))) (home-page "http://www-huber.embl.de/users/anders/HTSeq/") (synopsis "Analysing high-throughput sequencing data with Python") (description "HTSeq is a Python package that provides infrastructure to process data from high-throughput sequencing assays.") (license license:gpl3+))) (define-public htsjdk (package (name "htsjdk") (version "1.129") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htsjdk/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0asdk9b8jx2ij7yd6apg9qx03li8q7z3ml0qy2r2qczkra79y6fw")) (modules '((guix build utils))) ;; remove build dependency on git (snippet '(substitute* "build.xml" (("failifexecutionfails=\"true\"") "failifexecutionfails=\"false\""))))) (build-system gnu-build-system) (arguments `(#:modules ((srfi srfi-1) (guix build gnu-build-system) (guix build utils)) #:phases (alist-replace 'build (lambda _ (setenv "JAVA_HOME" (assoc-ref %build-inputs "jdk")) (zero? (system* "ant" "all" (string-append "-Ddist=" (assoc-ref %outputs "out") "/share/java/htsjdk/")))) (fold alist-delete %standard-phases '(configure install check))))) (native-inputs `(("ant" ,ant) ("jdk" ,icedtea6 "jdk"))) (home-page "http://samtools.github.io/htsjdk/") (synopsis "Java API for high-throughput sequencing data (HTS) formats") (description "HTSJDK is an implementation of a unified Java library for accessing common file formats, such as SAM and VCF, used for high-throughput sequencing (HTS) data. There are also an number of useful utilities for manipulating HTS data.") (license license:expat))) (define-public htslib (package (name "htslib") (version "1.2.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/samtools/htslib/releases/download/" version "/htslib-" version ".tar.bz2")) (sha256 (base32 "1c32ssscbnjwfw3dra140fq7riarp2x990qxybh34nr1p5r17nxx")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'patch-tests (lambda _ (substitute* "test/test.pl" (("/bin/bash") (which "bash"))) #t))))) (inputs `(("zlib" ,zlib))) (native-inputs `(("perl" ,perl))) (home-page "http://www.htslib.org") (synopsis "C library for reading/writing high-throughput sequencing data") (description "HTSlib is a C library for reading/writing high-throughput sequencing data. It also provides the bgzip, htsfile, and tabix utilities.") ;; Files under cram/ are released under the modified BSD license; ;; the rest is released under the Expat license (license (list license:expat license:bsd-3)))) (define-public idr (package (name "idr") (version "2.0.0") (source (origin (method url-fetch) (uri (string-append "https://github.com/nboley/idr/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1k3x44biak00aiv3hpm1yd6nn4hhp7n0qnbs3zh2q9sw7qr1qj5r")))) (build-system python-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'install 'wrap-program (lambda* (#:key inputs outputs #:allow-other-keys) (let* ((out (assoc-ref outputs "out")) (python-version (string-take (string-take-right (assoc-ref inputs "python") 5) 3)) (path (string-join (map (lambda (name) (string-append (assoc-ref inputs name) "/lib/python" python-version "/site-packages")) '("python-scipy" "python-numpy" "python-matplotlib")) ":"))) (wrap-program (string-append out "/bin/idr") `("PYTHONPATH" ":" prefix (,path)))) #t))))) (inputs `(("python-scipy" ,python-scipy) ("python-numpy" ,python-numpy) ("python-matplotlib" ,python-matplotlib))) (native-inputs `(("python-cython" ,python-cython) ("python-setuptools" ,python-setuptools))) (home-page "https://github.com/nboley/idr") (synopsis "Tool to measure the irreproducible discovery rate (IDR)") (description "The IDR (Irreproducible Discovery Rate) framework is a unified approach to measure the reproducibility of findings identified from replicate experiments and provide highly stable thresholds based on reproducibility.") (license license:gpl3+))) (define-public macs (package (name "macs") (version "2.1.0.20140616") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/M/MACS2/MACS2-" version ".tar.gz")) (sha256 (base32 "11lmiw6avqhwn75sn59g4lfkrr2kk20r3rgfbx9xfqb8rg9mi2n6")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; only compatible with Python 2.7 #:tests? #f)) ; no test target (inputs `(("python-numpy" ,python2-numpy))) (native-inputs `(("python-setuptools" ,python2-setuptools))) (home-page "http://github.com/taoliu/MACS/") (synopsis "Model based analysis for ChIP-Seq data") (description "MACS is an implementation of a ChIP-Seq analysis algorithm for identifying transcript factor binding sites named Model-based Analysis of ChIP-Seq (MACS). MACS captures the influence of genome complexity to evaluate the significance of enriched ChIP regions and it improves the spatial resolution of binding sites through combining the information of both sequencing tag position and orientation.") (license license:bsd-3))) (define-public mafft (package (name "mafft") (version "7.221") (source (origin (method url-fetch) (uri (string-append "http://mafft.cbrc.jp/alignment/software/mafft-" version "-without-extensions-src.tgz")) (file-name (string-append name "-" version ".tgz")) (sha256 (base32 "0xi7klbsgi049vsrk6jiwh9wfj3b770gz3c8c7zwij448v0dr73d")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no automated tests, though there are tests in the read me #:make-flags (let ((out (assoc-ref %outputs "out"))) (list (string-append "PREFIX=" out) (string-append "BINDIR=" (string-append out "/bin")))) #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "core") #t)) (add-after 'enter-dir 'patch-makefile (lambda _ ;; on advice from the MAFFT authors, there is no need to ;; distribute mafft-profile, mafft-distance, or ;; mafft-homologs.rb as they are too "specialised". (substitute* "Makefile" ;; remove mafft-homologs.rb from SCRIPTS (("^SCRIPTS = mafft mafft-homologs.rb") "SCRIPTS = mafft") ;; remove mafft-distance from PROGS (("^PROGS = dvtditr dndfast7 dndblast sextet5 mafft-distance") "PROGS = dvtditr dndfast7 dndblast sextet5") ;; remove mafft-profile from PROGS (("splittbfast disttbfast tbfast mafft-profile 2cl mccaskillwrap") "splittbfast disttbfast tbfast f2cl mccaskillwrap") (("^rm -f mafft-profile mafft-profile.exe") "#") (("^rm -f mafft-distance mafft-distance.exe") ")#") ;; do not install MAN pages in libexec folder (("^\t\\$\\(INSTALL\\) -m 644 \\$\\(MANPAGES\\) \ \\$\\(DESTDIR\\)\\$\\(LIBDIR\\)") "#")) #t)) (delete 'configure)))) (inputs `(("perl" ,perl))) (home-page "http://mafft.cbrc.jp/alignment/software/") (synopsis "Multiple sequence alignment program") (description "MAFFT offers a range of multiple alignment methods for nucleotide and protein sequences. For instance, it offers L-INS-i (accurate; for alignment of <~200 sequences) and FFT-NS-2 (fast; for alignment of <~30,000 sequences).") (license (license:non-copyleft "http://mafft.cbrc.jp/alignment/software/license.txt" "BSD-3 with different formatting")))) (define-public metabat (package (name "metabat") (version "0.26.1") (source (origin (method url-fetch) (uri (string-append "https://bitbucket.org/berkeleylab/metabat/get/" version ".tar.bz2")) (file-name (string-append name "-" version ".tar.bz2")) (sha256 (base32 "0vgrhbaxg4dkxyax2kbigak7w0arhqvw0szwp6gd9wmyilc44kfa")))) (build-system gnu-build-system) (arguments `(#:phases (modify-phases %standard-phases (add-after 'unpack 'fix-includes (lambda _ (substitute* "SConstruct" (("/include/bam/bam.h") "/include/samtools/bam.h")) (substitute* "src/BamUtils.h" (("^#include \"bam/bam\\.h\"") "#include \"samtools/bam.h\"") (("^#include \"bam/sam\\.h\"") "#include \"samtools/sam.h\"")) (substitute* "src/KseqReader.h" (("^#include \"bam/kseq\\.h\"") "#include \"samtools/kseq.h\"")) #t)) (add-after 'unpack 'fix-scons (lambda _ (substitute* "SConstruct" ; Do not distribute README (("^env\\.Install\\(idir_prefix, 'README\\.md'\\)") "")) #t)) (delete 'configure) (replace 'build (lambda* (#:key inputs outputs #:allow-other-keys) (mkdir (assoc-ref outputs "out")) (zero? (system* "scons" (string-append "PREFIX=" (assoc-ref outputs "out")) (string-append "HTSLIB_DIR=" (assoc-ref inputs "htslib")) (string-append "SAMTOOLS_DIR=" (assoc-ref inputs "samtools")) (string-append "BOOST_ROOT=" (assoc-ref inputs "boost")) "install")))) ;; check and install carried out during build phase (delete 'check) (delete 'install)))) (inputs `(("zlib" ,zlib) ("perl" ,perl) ("samtools" ,samtools) ("htslib" ,htslib) ("boost" ,boost))) (native-inputs `(("scons" ,scons))) (home-page "https://bitbucket.org/berkeleylab/metabat") (synopsis "Reconstruction of single genomes from complex microbial communities") (description "Grouping large genomic fragments assembled from shotgun metagenomic sequences to deconvolute complex microbial communities, or metagenome binning, enables the study of individual organisms and their interactions. MetaBAT is an automated metagenome binning software, which integrates empirical probabilistic distances of genome abundance and tetranucleotide frequency.") (license (license:non-copyleft "file://license.txt" "See license.txt in the distribution.")))) (define-public miso (package (name "miso") (version "0.5.3") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/m/misopy/misopy-" version ".tar.gz")) (sha256 (base32 "0x446867az8ir0z8c1vjqffkp0ma37wm4sylixnkhgawllzx8v5w")) (modules '((guix build utils))) (snippet '(substitute* "setup.py" ;; Use setuptools, or else the executables are not ;; installed. (("distutils.core") "setuptools") ;; use "gcc" instead of "cc" for compilation (("^defines") "cc.set_executables( compiler='gcc', compiler_so='gcc', linker_exe='gcc', linker_so='gcc -shared'); defines"))))) (build-system python-build-system) (arguments `(#:python ,python-2 ; only Python 2 is supported #:tests? #f)) ; no "test" target (inputs `(("samtools" ,samtools) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-scipy" ,python2-scipy) ("python-matplotlib" ,python2-matplotlib))) (native-inputs `(("python-mock" ,python2-mock) ;for tests ("python-pytz" ,python2-pytz) ;for tests ("python-setuptools" ,python2-setuptools))) (home-page "http://genes.mit.edu/burgelab/miso/index.html") (synopsis "Mixture of Isoforms model for RNA-Seq isoform quantitation") (description "MISO (Mixture-of-Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA-Seq data, and identifies differentially regulated isoforms or exons across samples. By modeling the generative process by which reads are produced from isoforms in RNA-Seq, the MISO model uses Bayesian inference to compute the probability that a read originated from a particular isoform.") (license license:gpl2))) (define-public orfm (package (name "orfm") (version "0.4.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/wwood/OrfM/releases/download/v" version "/orfm-" version ".tar.gz")) (sha256 (base32 "05fmw145snk646ly076zby0fjav0k7ysbclck5d4s9pmgcfpijc2")))) (build-system gnu-build-system) (inputs `(("zlib" ,zlib))) (synopsis "Simple and not slow open reading frame (ORF) caller") (description "An ORF caller finds stretches of DNA that when translated are not interrupted by stop codons. OrfM finds and prints these ORFs.") (home-page "https://github.com/wwood/OrfM") (license license:lgpl3+))) (define-public python2-pbcore (package (name "python2-pbcore") (version "0.9.3") (source (origin (method url-fetch) (uri (string-append "https://github.com/PacificBiosciences/pbcore/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1z46rwjac93jm87cbj2zgjg6qvsgs65140wkbbxsvxps7ai4pm09")))) (build-system python-build-system) (arguments `(#:python ,python-2)) ; pbcore requires Python 2.7 (inputs `(("python-cython" ,python2-cython) ("python-numpy" ,python2-numpy) ("python-pysam" ,python2-pysam) ("python-h5py" ,python2-h5py))) (native-inputs `(("python-setuptools" ,python2-setuptools))) (home-page "http://pacificbiosciences.github.io/pbcore/") (synopsis "Library for reading and writing PacBio data files") (description "The pbcore package provides Python APIs for interacting with PacBio data files and writing bioinformatics applications.") (license license:bsd-3))) (define-public python2-warpedlmm (package (name "python2-warpedlmm") (version "0.21") (source (origin (method url-fetch) (uri (string-append "https://pypi.python.org/packages/source/W/WarpedLMM/WarpedLMM-" version ".zip")) (sha256 (base32 "1agfz6zqa8nc6cw47yh0s3y14gkpa9wqazwcj7mwwj3ffnw39p3j")))) (build-system python-build-system) (arguments `(#:python ,python-2 ; requires Python 2.7 #:phases (modify-phases %standard-phases (add-after 'install 'remove-bin-directory (lambda* (#:key outputs #:allow-other-keys) ;; The "bin" directory only contains wrappers for running ;; the module tests. They are not needed after the ;; "check" phase. (delete-file-recursively (string-append (assoc-ref outputs "out") "/bin")) #t))))) (propagated-inputs `(("python-scipy" ,python2-scipy) ("python-numpy" ,python2-numpy) ("python-matplotlib" ,python2-matplotlib) ("python-fastlmm" ,python2-fastlmm) ("python-pandas" ,python2-pandas) ("python-pysnptools" ,python2-pysnptools))) (native-inputs `(("python-setuptools" ,python2-setuptools) ("python-mock" ,python2-mock) ("python-nose" ,python2-nose) ("unzip" ,unzip))) (home-page "https://github.com/PMBio/warpedLMM") (synopsis "Implementation of warped linear mixed models") (description "WarpedLMM is a Python implementation of the warped linear mixed model, which automatically learns an optimal warping function (or transformation) for the phenotype as it models the data.") (license license:asl2.0))) (define-public pbtranscript-tofu (let ((commit "c7bbd5472")) (package (name "pbtranscript-tofu") (version (string-append "0.4.1." commit)) (source (origin (method git-fetch) (uri (git-reference (url "https://github.com/PacificBiosciences/cDNA_primer.git") (commit commit))) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "148xkzi689c49g6fdhckp6mnmj2qhjdf1j4wifm6ja7ij95d7fxx")))) (build-system python-build-system) (arguments `(#:python ,python-2 ;; With standard flags, the install phase attempts to create a zip'd ;; egg file, and fails with an error: 'ZIP does not support timestamps ;; before 1980' #:configure-flags '("--single-version-externally-managed" "--record=pbtranscript-tofu.txt") #:phases (alist-cons-after 'unpack 'enter-directory-and-clean-up (lambda _ (chdir "pbtranscript-tofu/pbtranscript/") ;; Delete clutter (delete-file-recursively "dist/") (delete-file-recursively "build/") (delete-file-recursively "setuptools_cython-0.2.1-py2.6.egg/") (delete-file-recursively "pbtools.pbtranscript.egg-info") (delete-file "Cython-0.20.1.tar.gz") (delete-file "setuptools_cython-0.2.1-py2.7.egg") (delete-file "setuptools_cython-0.2.1.tar.gz") (delete-file "setup.cfg") (for-each delete-file (find-files "." "\\.so$")) ;; files should be writable for install phase (for-each (lambda (f) (chmod f #o755)) (find-files "." "\\.py$"))) %standard-phases))) (inputs `(("python-cython" ,python2-cython) ("python-numpy" ,python2-numpy) ("python-bx-python" ,python2-bx-python) ("python-networkx" ,python2-networkx) ("python-scipy" ,python2-scipy) ("python-pbcore" ,python2-pbcore))) (native-inputs `(("python-nose" ,python2-nose) ("python-setuptools" ,python2-setuptools))) (home-page "https://github.com/PacificBiosciences/cDNA_primer") (synopsis "Analyze transcriptome data generated with the Iso-Seq protocol") (description "pbtranscript-tofu contains scripts to analyze transcriptome data generated using the PacBio Iso-Seq protocol.") (license license:bsd-3)))) (define-public prodigal (package (name "prodigal") (version "2.6.2") (source (origin (method url-fetch) (uri (string-append "https://github.com/hyattpd/Prodigal/archive/v" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "0m8sb0fg6lmxrlpzna0am6svbnlmd3dckrhgzxxgb3gxr5fyj284")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no check target #:make-flags (list (string-append "INSTALLDIR=" (assoc-ref %outputs "out") "/bin")) #:phases (modify-phases %standard-phases (delete 'configure)))) (home-page "http://prodigal.ornl.gov") (synopsis "Protein-coding gene prediction for Archaea and Bacteria") (description "Prodigal runs smoothly on finished genomes, draft genomes, and metagenomes, providing gene predictions in GFF3, Genbank, or Sequin table format. It runs quickly, in an unsupervised fashion, handles gaps, handles partial genes, and identifies translation initiation sites.") (license license:gpl3+))) (define-public rsem (package (name "rsem") (version "1.2.20") (source (origin (method url-fetch) (uri (string-append "http://deweylab.biostat.wisc.edu/rsem/src/rsem-" version ".tar.gz")) (sha256 (base32 "0nzdc0j0hjllhsd5f2xli95dafm3nawskigs140xzvjk67xh0r9q")) (patches (list (search-patch "rsem-makefile.patch"))) (modules '((guix build utils))) (snippet '(begin ;; remove bundled copy of boost (delete-file-recursively "boost") #t)))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases ;; No "configure" script. ;; Do not build bundled samtools library. (replace 'configure (lambda _ (substitute* "Makefile" (("^all : sam/libbam.a") "all : ")) #t)) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let* ((out (string-append (assoc-ref outputs "out"))) (bin (string-append out "/bin/")) (perl (string-append out "/lib/perl5/site_perl"))) (mkdir-p bin) (mkdir-p perl) (for-each (lambda (file) (copy-file file (string-append bin (basename file)))) (find-files "." "rsem-.*")) (copy-file "rsem_perl_utils.pm" (string-append perl "/rsem_perl_utils.pm"))) #t)) (add-after 'install 'wrap-program (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) (for-each (lambda (prog) (wrap-program (string-append out "/bin/" prog) `("PERL5LIB" ":" prefix (,(string-append out "/lib/perl5/site_perl"))))) '("rsem-plot-transcript-wiggles" "rsem-calculate-expression" "rsem-generate-ngvector" "rsem-run-ebseq" "rsem-prepare-reference"))) #t))))) (inputs `(("boost" ,boost) ("ncurses" ,ncurses) ("r" ,r) ("perl" ,perl) ("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "http://deweylab.biostat.wisc.edu/rsem/") (synopsis "Estimate gene expression levels from RNA-Seq data") (description "RSEM is a software package for estimating gene and isoform expression levels from RNA-Seq data. The RSEM package provides a user-friendly interface, supports threads for parallel computation of the EM algorithm, single-end and paired-end read data, quality scores, variable-length reads and RSPD estimation. In addition, it provides posterior mean and 95% credibility interval estimates for expression levels. For visualization, it can generate BAM and Wiggle files in both transcript-coordinate and genomic-coordinate.") (license license:gpl3+))) (define-public rseqc (package (name "rseqc") (version "2.6.1") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/rseqc/" version "/RSeQC-" version ".tar.gz")) (sha256 (base32 "15ly0254yi032qzkdplg00q144qfdsd986gh62829rl5bkxhj330")) (modules '((guix build utils))) (snippet '(begin ;; remove bundled copy of pysam (delete-file-recursively "lib/pysam") (substitute* "setup.py" ;; remove dependency on outdated "distribute" module (("^from distribute_setup import use_setuptools") "") (("^use_setuptools\\(\\)") "") ;; do not use bundled copy of pysam (("^have_pysam = False") "have_pysam = True")))))) (build-system python-build-system) (arguments `(#:python ,python-2)) (inputs `(("python-cython" ,python2-cython) ("python-pysam" ,python2-pysam) ("python-numpy" ,python2-numpy) ("python-setuptools" ,python2-setuptools) ("zlib" ,zlib))) (native-inputs `(("python-nose" ,python2-nose))) (home-page "http://rseqc.sourceforge.net/") (synopsis "RNA-seq quality control package") (description "RSeQC provides a number of modules that can comprehensively evaluate high throughput sequence data, especially RNA-seq data. Some basic modules inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while RNA-seq specific modules evaluate sequencing saturation, mapped reads distribution, coverage uniformity, strand specificity, etc.") (license license:gpl3+))) (define-public samtools (package (name "samtools") (version "1.2") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "1akdqb685pk9xk1nb6sa9aq8xssjjhvvc06kp4cpdqvz2157l3j2")))) (build-system gnu-build-system) (arguments `(;; There are 87 test failures when building on non-64-bit architectures ;; due to invalid test data. This has since been fixed upstream (see ;; ), but as there has ;; not been a new release we disable the tests for all non-64-bit ;; systems. #:tests? ,(string=? (or (%current-system) (%current-target-system)) "x86_64-linux") #:modules ((ice-9 ftw) (ice-9 regex) (guix build gnu-build-system) (guix build utils)) #:make-flags (list "LIBCURSES=-lncurses" (string-append "prefix=" (assoc-ref %outputs "out"))) #:phases (alist-cons-after 'unpack 'patch-tests (lambda* (#:key inputs #:allow-other-keys) (let ((bash (assoc-ref inputs "bash"))) (substitute* "test/test.pl" ;; The test script calls out to /bin/bash (("/bin/bash") (string-append bash "/bin/bash")) ;; There are two failing tests upstream relating to the "stats" ;; subcommand in test_usage_subcommand ("did not have Usage" ;; and "usage did not mention samtools stats"), so we disable ;; them. (("(test_usage_subcommand\\(.*\\);)" cmd) (string-append "unless ($subcommand eq 'stats') {" cmd "};"))))) (alist-cons-after 'install 'install-library (lambda* (#:key outputs #:allow-other-keys) (let ((lib (string-append (assoc-ref outputs "out") "/lib"))) (mkdir-p lib) (copy-file "libbam.a" (string-append lib "/libbam.a")))) (alist-cons-after 'install 'install-headers (lambda* (#:key outputs #:allow-other-keys) (let ((include (string-append (assoc-ref outputs "out") "/include/samtools/"))) (mkdir-p include) (for-each (lambda (file) (copy-file file (string-append include (basename file)))) (scandir "." (lambda (name) (string-match "\\.h$" name)))) #t)) (alist-delete 'configure %standard-phases)))))) (native-inputs `(("pkg-config" ,pkg-config))) (inputs `(("ncurses" ,ncurses) ("perl" ,perl) ("python" ,python) ("zlib" ,zlib))) (home-page "http://samtools.sourceforge.net") (synopsis "Utilities to efficiently manipulate nucleotide sequence alignments") (description "Samtools implements various utilities for post-processing nucleotide sequence alignments in the SAM, BAM, and CRAM formats, including indexing, variant calling (in conjunction with bcftools), and a simple alignment viewer.") (license license:expat))) (define-public samtools-0.1 ;; This is the most recent version of the 0.1 line of samtools. The input ;; and output formats differ greatly from that used and produced by samtools ;; 1.x and is still used in many bioinformatics pipelines. (package (inherit samtools) (version "0.1.19") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/samtools/" version "/samtools-" version ".tar.bz2")) (sha256 (base32 "1m33xsfwz0s8qi45lylagfllqg7fphf4dr0780rsvw75av9wk06h")))) (arguments (substitute-keyword-arguments (package-arguments samtools) ((#:tests? tests) #f) ;no "check" target ((#:phases phases) `(modify-phases ,phases (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin"))) (mkdir-p bin) (copy-file "samtools" (string-append bin "/samtools"))))) (delete 'patch-tests))))))) (define-public ngs-sdk (package (name "ngs-sdk") (version "1.1.1") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/ngs/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1x58gpm574n0xmk2a98gmikbgycq78ia0bvnb42k5ck34fmd5v8y")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (alist-replace 'configure (lambda* (#:key outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" out))))) (alist-cons-after 'unpack 'enter-dir (lambda _ (chdir "ngs-sdk") #t) %standard-phases)))) (native-inputs `(("perl" ,perl))) (home-page "https://github.com/ncbi/ngs") (synopsis "API for accessing Next Generation Sequencing data") (description "NGS is a domain-specific API for accessing reads, alignments and pileups produced from Next Generation Sequencing. The API itself is independent from any particular back-end implementation, and supports use of multiple back-ends simultaneously.") (license license:public-domain))) (define-public ngs-java (package (inherit ngs-sdk) (name "ngs-java") (arguments `(,@(substitute-keyword-arguments `(#:modules ((guix build gnu-build-system) (guix build utils) (srfi srfi-1) (srfi srfi-26)) ,@(package-arguments ngs-sdk)) ((#:phases phases) `(alist-cons-after 'enter-dir 'fix-java-symlink-installation (lambda _ ;; Only replace the version suffix, not the version number in ;; the directory name. Reported here: ;; https://github.com/ncbi/ngs/pull/4 (substitute* "Makefile.java" (((string-append "\\$\\(subst " "(\\$\\(VERSION[^\\)]*\\))," "(\\$\\([^\\)]+\\))," "(\\$\\([^\\)]+\\)|\\$\\@)" "\\)") _ pattern replacement target) (string-append "$(patsubst " "%" pattern "," "%" replacement "," target ")")))) (alist-replace 'enter-dir (lambda _ (chdir "ngs-java") #t) ,phases)))))) (inputs `(("jdk" ,icedtea6 "jdk") ("ngs-sdk" ,ngs-sdk))) (synopsis "Java bindings for NGS SDK"))) (define-public ncbi-vdb (package (name "ncbi-vdb") (version "2.4.5-5") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/ncbi-vdb/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "1cj8nk6if8sqagv20vx36v566fdvhcaadf0x1ycnbgql6chbs6vy")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (alist-replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) (let ((out (assoc-ref outputs "out"))) ;; Only replace the version suffix, not the version number in the ;; directory name; fixed in commit 4dbba5c6a809 (no release yet). (substitute* "setup/konfigure.perl" (((string-append "\\$\\(subst " "(\\$\\(VERSION[^\\)]*\\))," "(\\$\\([^\\)]+\\))," "(\\$\\([^\\)]+\\)|\\$\\@)" "\\)") _ pattern replacement target) (string-append "$(patsubst " "%" pattern "," "%" replacement "," target ")"))) ;; Override include path for libmagic (substitute* "setup/package.prl" (("name => 'magic', Include => '/usr/include'") (string-append "name=> 'magic', Include => '" (assoc-ref inputs "libmagic") "/include" "'"))) ;; Install kdf5 library (needed by sra-tools) (substitute* "build/Makefile.install" (("LIBRARIES_TO_INSTALL =") "LIBRARIES_TO_INSTALL = kdf5.$(VERSION_LIBX) kdf5.$(VERSION_SHLX)")) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-xml2-prefix=" (assoc-ref inputs "libxml2")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-ngs-java-prefix=" (assoc-ref inputs "ngs-java")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5")))))) (alist-cons-after 'install 'install-interfaces (lambda* (#:key outputs #:allow-other-keys) ;; Install interface libraries. On i686 the interface libraries ;; are installed to "linux/gcc/i386", so we need to use the Linux ;; architecture name ("i386") instead of the target system prefix ;; ("i686"). (mkdir (string-append (assoc-ref outputs "out") "/ilib")) (copy-recursively (string-append "build/ncbi-vdb/linux/gcc/" ,(system->linux-architecture (or (%current-target-system) (%current-system))) "/rel/ilib") (string-append (assoc-ref outputs "out") "/ilib")) ;; Install interface headers (copy-recursively "interfaces" (string-append (assoc-ref outputs "out") "/include"))) %standard-phases)))) (inputs `(("libxml2" ,libxml2) ("ngs-sdk" ,ngs-sdk) ("ngs-java" ,ngs-java) ("libmagic" ,file) ("hdf5" ,hdf5))) (native-inputs `(("perl" ,perl))) (home-page "https://github.com/ncbi/ncbi-vdb") (synopsis "Database engine for genetic information") (description "The NCBI-VDB library implements a highly compressed columnar data warehousing engine that is most often used to store genetic information. Databases are stored in a portable image within the file system, and can be accessed/downloaded on demand across HTTP.") (license license:public-domain))) (define-public plink (package (name "plink") (version "1.07") (source (origin (method url-fetch) (uri (string-append "http://pngu.mgh.harvard.edu/~purcell/plink/dist/plink-" version "-src.zip")) (sha256 (base32 "0as8gxm4pjyc8dxmm1sl873rrd7wn5qs0l29nqfnl31x8i467xaa")) (patches (list (search-patch "plink-1.07-unclobber-i.patch"))))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no "check" target #:make-flags (list (string-append "LIB_LAPACK=" (assoc-ref %build-inputs "lapack") "/lib/liblapack.so") "WITH_LAPACK=1" "FORCE_DYNAMIC=1" ;; disable phoning home "WITH_WEBCHECK=") #:phases (modify-phases %standard-phases ;; no "configure" script (delete 'configure) (replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (copy-file "plink" (string-append bin "plink")) #t)))))) (inputs `(("zlib" ,zlib) ("lapack" ,lapack))) (native-inputs `(("unzip" ,unzip))) (home-page "http://pngu.mgh.harvard.edu/~purcell/plink/") (synopsis "Whole genome association analysis toolset") (description "PLINK is a whole genome association analysis toolset, designed to perform a range of basic, large-scale analyses in a computationally efficient manner. The focus of PLINK is purely on analysis of genotype/phenotype data, so there is no support for steps prior to this (e.g. study design and planning, generating genotype or CNV calls from raw data). Through integration with gPLINK and Haploview, there is some support for the subsequent visualization, annotation and storage of results.") ;; Code is released under GPLv2, except for fisher.h, which is under ;; LGPLv2.1+ (license (list license:gpl2 license:lgpl2.1+)))) (define-public preseq (package (name "preseq") (version "1.0.2") (source (origin (method url-fetch) (uri (string-append "http://smithlabresearch.org/downloads/preseq-" version ".tar.bz2")) (sha256 (base32 "0r7sw07p6nv8ygvc17gd78lisbw5336v3vhs86b5wv8mw3pwqksc")) (patches (list (search-patch "preseq-1.0.2-install-to-PREFIX.patch") (search-patch "preseq-1.0.2-link-with-libbam.patch"))) (modules '((guix build utils))) (snippet ;; Remove bundled samtools. '(delete-file-recursively "preseq-master/samtools")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:phases (modify-phases %standard-phases (add-after 'unpack 'enter-dir (lambda _ (chdir "preseq-master") #t)) (add-after 'enter-dir 'use-samtools-headers (lambda _ (substitute* '("smithlab_cpp/SAM.cpp" "smithlab_cpp/SAM.hpp") (("sam.h") "samtools/sam.h")) #t)) (delete 'configure)) #:make-flags (list (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "LIBBAM=" (assoc-ref %build-inputs "samtools") "/lib/libbam.a")))) (inputs `(("gsl" ,gsl) ("samtools" ,samtools-0.1) ("zlib" ,zlib))) (home-page "http://smithlabresearch.org/software/preseq/") (synopsis "Program for analyzing library complexity") (description "The preseq package is aimed at predicting and estimating the complexity of a genomic sequencing library, equivalent to predicting and estimating the number of redundant reads from a given sequencing depth and how many will be expected from additional sequencing using an initial sequencing experiment. The estimates can then be used to examine the utility of further sequencing, optimize the sequencing depth, or to screen multiple libraries to avoid low complexity samples.") (license license:gpl3+))) (define-public sra-tools (package (name "sra-tools") (version "2.4.5-5") (source (origin (method url-fetch) (uri (string-append "https://github.com/ncbi/sra-tools/archive/" version ".tar.gz")) (file-name (string-append name "-" version ".tar.gz")) (sha256 (base32 "11nrnvz7a012f4iryf0wiwrid0h111grsfxbxa9j51h3f2xbvgns")))) (build-system gnu-build-system) (arguments `(#:parallel-build? #f ; not supported #:tests? #f ; no "check" target #:phases (alist-replace 'configure (lambda* (#:key inputs outputs #:allow-other-keys) ;; The build system expects a directory containing the sources and ;; raw build output of ncbi-vdb, including files that are not ;; installed. Since we are building against an installed version of ;; ncbi-vdb, the following modifications are needed. (substitute* "setup/konfigure.perl" ;; Make the configure script look for the "ilib" directory of ;; "ncbi-vdb" without first checking for the existence of a ;; matching library in its "lib" directory. (("^ my \\$f = File::Spec->catdir\\(\\$libdir, \\$lib\\);") "my $f = File::Spec->catdir($ilibdir, $ilib);") ;; Look for interface libraries in ncbi-vdb's "ilib" directory. (("my \\$ilibdir = File::Spec->catdir\\(\\$builddir, 'ilib'\\);") "my $ilibdir = File::Spec->catdir($dir, 'ilib');")) ;; The 'configure' script doesn't recognize things like ;; '--enable-fast-install'. (zero? (system* "./configure" (string-append "--build-prefix=" (getcwd) "/build") (string-append "--prefix=" (assoc-ref outputs "out")) (string-append "--debug") (string-append "--with-fuse-prefix=" (assoc-ref inputs "fuse")) (string-append "--with-magic-prefix=" (assoc-ref inputs "libmagic")) ;; TODO: building with libxml2 fails with linker errors ;; (string-append "--with-xml2-prefix=" ;; (assoc-ref inputs "libxml2")) (string-append "--with-ncbi-vdb-sources=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ncbi-vdb-build=" (assoc-ref inputs "ncbi-vdb")) (string-append "--with-ngs-sdk-prefix=" (assoc-ref inputs "ngs-sdk")) (string-append "--with-hdf5-prefix=" (assoc-ref inputs "hdf5"))))) %standard-phases))) (native-inputs `(("perl" ,perl))) (inputs `(("ngs-sdk" ,ngs-sdk) ("ncbi-vdb" ,ncbi-vdb) ("libmagic" ,file) ("fuse" ,fuse) ("hdf5" ,hdf5) ("zlib" ,zlib))) (home-page "http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software") (synopsis "Tools and libraries for reading and writing sequencing data") (description "The SRA Toolkit from NCBI is a collection of tools and libraries for reading of sequencing files from the Sequence Read Archive (SRA) database and writing files into the .sra format.") (license license:public-domain))) (define-public seqan (package (name "seqan") (version "1.4.2") (source (origin (method url-fetch) (uri (string-append "http://packages.seqan.de/seqan-library/" "seqan-library-" version ".tar.bz2")) (sha256 (base32 "05s3wrrwn50f81aklfm65i4a749zag1vr8z03k21xm0pdxy47yvp")))) ;; The documentation is 7.8MB and the includes are 3.6MB heavy, so it ;; makes sense to split the outputs. (outputs '("out" "doc")) (build-system trivial-build-system) (arguments `(#:modules ((guix build utils)) #:builder (begin (use-modules (guix build utils)) (let ((tar (assoc-ref %build-inputs "tar")) (bzip (assoc-ref %build-inputs "bzip2")) (out (assoc-ref %outputs "out")) (doc (assoc-ref %outputs "doc"))) (setenv "PATH" (string-append tar "/bin:" bzip "/bin")) (system* "tar" "xvf" (assoc-ref %build-inputs "source")) (chdir (string-append "seqan-library-" ,version)) (copy-recursively "include" (string-append out "/include")) (copy-recursively "share" (string-append doc "/share")))))) (native-inputs `(("source" ,source) ("tar" ,tar) ("bzip2" ,bzip2))) (home-page "http://www.seqan.de") (synopsis "Library for nucleotide sequence analysis") (description "SeqAn is a C++ library of efficient algorithms and data structures for the analysis of sequences with the focus on biological data. It contains algorithms and data structures for string representation and their manipulation, online and indexed string search, efficient I/O of bioinformatics file formats, sequence alignment, and more.") (license license:bsd-3))) (define-public star (package (name "star") (version "2.4.2a") (source (origin (method url-fetch) (uri (string-append "https://github.com/alexdobin/STAR/archive/STAR_" version ".tar.gz")) (sha256 (base32 "1c3rnm7r5l0kl3d04gl1g7938xqf1c2l0mla87rlplqg1hcns5mc")) (modules '((guix build utils))) (snippet '(substitute* "source/Makefile" (("/bin/rm") "rm"))))) (build-system gnu-build-system) (arguments '(#:tests? #f ;no check target #:make-flags '("STAR") #:phases (alist-cons-after 'unpack 'enter-source-dir (lambda _ (chdir "source")) (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (copy-file "STAR" (string-append bin "STAR")))) (alist-delete 'configure %standard-phases))))) (native-inputs `(("vim" ,vim))) ; for xxd (inputs `(("zlib" ,zlib))) (home-page "https://github.com/alexdobin/STAR") (synopsis "Universal RNA-seq aligner") (description "The Spliced Transcripts Alignment to a Reference (STAR) software is based on a previously undescribed RNA-seq alignment algorithm that uses sequential maximum mappable seed search in uncompressed suffix arrays followed by seed clustering and stitching procedure. In addition to unbiased de novo detection of canonical junctions, STAR can discover non-canonical splices and chimeric (fusion) transcripts, and is also capable of mapping full-length RNA sequences.") ;; STAR is licensed under GPLv3 or later; htslib is MIT-licensed. (license license:gpl3+))) (define-public subread (package (name "subread") (version "1.4.6-p2") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/subread/subread-" version "-source.tar.gz")) (sha256 (base32 "06sv9mpcsdj6p68y15d6gi70lca3lxmzk0dn61hg0kfsa7rxmsr3")))) (build-system gnu-build-system) (arguments `(#:tests? #f ;no "check" target #:make-flags '("-f" "Makefile.Linux") #:phases (alist-cons-after 'unpack 'enter-dir (lambda _ (chdir "src") #t) (alist-replace 'install (lambda* (#:key outputs #:allow-other-keys) (let ((bin (string-append (assoc-ref outputs "out") "/bin/"))) (mkdir-p bin) (copy-recursively "../bin" bin))) ;; no "configure" script (alist-delete 'configure %standard-phases))))) (inputs `(("zlib" ,zlib))) (home-page "http://bioinf.wehi.edu.au/subread-package/") (synopsis "Tool kit for processing next-gen sequencing data") (description "The subread package contains the following tools: subread aligner, a general-purpose read aligner; subjunc aligner: detecting exon-exon junctions and mapping RNA-seq reads; featureCounts: counting mapped reads for genomic features; exactSNP: a SNP caller that discovers SNPs by testing signals against local background noises.") (license license:gpl3+))) (define-public vcftools (package (name "vcftools") (version "0.1.12b") (source (origin (method url-fetch) (uri (string-append "mirror://sourceforge/vcftools/vcftools_" version ".tar.gz")) (sha256 (base32 "148al9h7f8g8my2qdnpax51kdd2yjrivlx6frvakf4lz5r8j88wx")))) (build-system gnu-build-system) (arguments `(#:tests? #f ; no "check" target #:make-flags (list "CFLAGS=-O2" ; override "-m64" flag (string-append "PREFIX=" (assoc-ref %outputs "out")) (string-append "MANDIR=" (assoc-ref %outputs "out") "/share/man/man1")) #:phases (alist-cons-after 'unpack 'patch-manpage-install (lambda _ (substitute* "Makefile" (("cp \\$\\{PREFIX\\}/cpp/vcftools.1") "cp ./cpp/vcftools.1"))) (alist-delete 'configure %standard-phases)))) (inputs `(("perl" ,perl) ("zlib" ,zlib))) (home-page "http://vcftools.sourceforge.net/") (synopsis "Tools for working with VCF files") (description "VCFtools is a program package designed for working with VCF files, such as those generated by the 1000 Genomes Project. The aim of VCFtools is to provide easily accessible methods for working with complex genetic variation data in the form of VCF files.") ;; The license is declared as LGPLv3 in the README and ;; at http://vcftools.sourceforge.net/license.html (license license:lgpl3))) (define-public bio-locus (package (name "bio-locus") (version "0.0.7") (source (origin (method url-fetch) (uri (rubygems-uri "bio-locus" version)) (sha256 (base32 "02vmrxyimkj9sahsp4zhfhnmbvz6dbbqz1y01vglf8cbwvkajfl0")))) (build-system ruby-build-system) (native-inputs `(("ruby-rspec" ,ruby-rspec))) (synopsis "Tool for fast querying of genome locations") (description "Bio-locus is a tabix-like tool for fast querying of genome locations. Many file formats in bioinformatics contain records that start with a chromosome name and a position for a SNP, or a start-end position for indels. Bio-locus allows users to store this chr+pos or chr+pos+alt information in a database.") (home-page "https://github.com/pjotrp/bio-locus") (license license:expat))) (define-public r-qtl (package (name "r-qtl") (version "1.37-11") (source (origin (method url-fetch) (uri (string-append "mirror://cran/src/contrib/qtl_" version ".tar.gz")) (sha256 (base32 "0h20d36mww7ljp51pfs66xq33yq4b4fwq9nsh02dpmfhlaxgx1xi")))) (build-system r-build-system) (home-page "http://www.rqtl.org") (synopsis "Tools for Analyzing QTL Experiments") (description "Bio-statistics library for analysis of experimental crosses to identify genes (called quantitative trait loci, QTLs) contributing to variation in quantitative traits.") (license license:gpl3+)))