diff --git a/guix/build/go-build-system.scm b/guix/build/go-build-system.scm
index fc5ee39c8d..a6b9397d35 100644
--- a/guix/build/go-build-system.scm
+++ b/guix/build/go-build-system.scm
@@ -137,6 +137,9 @@ dependencies, so it should be self-contained."
   ;; Using the current working directory as GOPATH makes it easier for packagers
   ;; who need to manipulate the unpacked source code.
   (setenv "GOPATH" (string-append (getcwd) ":" (getenv "GOPATH")))
+  ;; Unconditionally set the -trimpath option to avoid spurious store references
+  ;; from having multiple GOPATH entries.  See <https://bugs.gnu.org/33620>.
+  (setenv "GOFLAGS" "-trimpath")
   ;; Go 1.13 uses go modules by default. The go build system does not
   ;; currently support modules, so turn modules off to continue using the old
   ;; GOPATH behavior.
@@ -188,8 +191,6 @@ unpacking."
       (apply invoke "go" "install"
               "-v" ; print the name of packages as they are compiled
               "-x" ; print each command as it is invoked
-              ;; Trim store references from the compiled binaries.
-              "-trimpath"
               ;; Respectively, strip the symbol table and debug
               ;; information, and the DWARF symbol table.
               "-ldflags=-s -w"
@@ -202,6 +203,9 @@ unpacking."
 ;; Can this also install commands???
 (define* (check #:key tests? import-path #:allow-other-keys)
   "Run the tests for the package named by IMPORT-PATH."
+  ;; Remove the global -trimpath option because it can break some test
+  ;; suites.
+  (unsetenv "GOFLAGS")
   (when tests?
     (invoke "go" "test" import-path))
   #t)