From mboxrd@z Thu Jan 1 00:00:00 1970 Path: news.gmane.org!not-for-mail From: =?GB2312?B?sb7UqtX90fQ=?= Newsgroups: gmane.emacs.bugs,gmane.spam.detected Subject: (no subject) Date: Tue, 19 Apr 2005 00:06:12 +0800 Message-ID: Reply-To: vpr@agtc.com.cn NNTP-Posting-Host: main.gmane.org Content-Type: multipart/mixed; boundary="===============0384674122==" X-Trace: sea.gmane.org 1121200459 6766 80.91.229.2 (12 Jul 2005 20:34:19 GMT) X-Complaints-To: usenet@sea.gmane.org NNTP-Posting-Date: Tue, 12 Jul 2005 20:34:19 +0000 (UTC) Original-X-From: bug-gnu-emacs-bounces+geb-bug-gnu-emacs=m.gmane.org@gnu.org Tue Jul 12 22:34:08 2005 Return-path: Original-Received: from lists.gnu.org ([199.232.76.165]) by ciao.gmane.org with esmtp (Exim 4.43) id 1DsRQp-0003XL-Uw for geb-bug-gnu-emacs@m.gmane.org; Tue, 12 Jul 2005 22:32:44 +0200 Original-Received: from localhost ([127.0.0.1] helo=lists.gnu.org) by lists.gnu.org with esmtp (Exim 4.43) id 1DsRST-0004e4-Cz for geb-bug-gnu-emacs@m.gmane.org; Tue, 12 Jul 2005 16:34:25 -0400 Original-Received: from mailman by lists.gnu.org with tmda-scanned (Exim 4.43) id 1DONYk-000582-DD for bug-gnu-emacs@gnu.org; Wed, 20 Apr 2005 18:20:38 -0400 Original-Received: from exim by lists.gnu.org with spam-scanned (Exim 4.43) id 1DONYg-00055t-1N for bug-gnu-emacs@gnu.org; Wed, 20 Apr 2005 18:20:36 -0400 Original-Received: from [199.232.76.173] (helo=monty-python.gnu.org) by lists.gnu.org with esmtp (Exim 4.43) id 1DONYf-0004V5-3S for bug-gnu-emacs@gnu.org; Wed, 20 Apr 2005 18:20:33 -0400 Original-Received: from [199.232.41.67] (helo=mx20.gnu.org) by monty-python.gnu.org with esmtp (TLS-1.0:RSA_ARCFOUR_SHA:16) (Exim 4.34) id 1DONZx-0007XZ-6D for bug-gnu-emacs@gnu.org; Wed, 20 Apr 2005 18:21:53 -0400 Original-Received: from [61.49.160.100] (helo=agtc.com.cn) by mx20.gnu.org with esmtp (Exim 4.34) id 1DNYlI-0003pS-Os for bug-gnu-emacs@gnu.org; Mon, 18 Apr 2005 12:06:31 -0400 Original-To: bug-gnu-emacs@gnu.org X-Priority: 3 X-Mailer: Microsoft Outlook Express 6.00.2600.0000 X-BeenThere: bug-gnu-emacs@gnu.org X-Mailman-Version: 2.1.5 Precedence: list List-Id: "Bug reports for GNU Emacs, the Swiss army knife of text editors" List-Unsubscribe: , List-Archive: List-Post: List-Help: List-Subscribe: , Original-Sender: bug-gnu-emacs-bounces+geb-bug-gnu-emacs=m.gmane.org@gnu.org Errors-To: bug-gnu-emacs-bounces+geb-bug-gnu-emacs=m.gmane.org@gnu.org X-Spam-Report: 6.2 points; * -0.0 SPF_HELO_PASS SPF: HELO matches SPF record * -0.0 SPF_PASS SPF: sender matches SPF record * 0.0 HTML_MESSAGE BODY: HTML included in message * 2.0 BAYES_80 BODY: Bayesian spam probability is 80 to 95% * [score: 0.9055] * 0.1 HTML_TAG_EXIST_TBODY BODY: HTML has "tbody" tag * 0.0 HTML_90_100 BODY: Message is 90% to 100% HTML * 0.1 MIME_HEADER_CTYPE_ONLY 'Content-Type' found without required MIME headers * 3.9 FORGED_MUA_OUTLOOK Forged mail pretending to be from MS Outlook Xref: news.gmane.org gmane.emacs.bugs:12461 gmane.spam.detected:690098 X-Report-Unspam: http://unspam.gmane.org/gmane.emacs.bugs:12461 --===============0384674122== Content-Type: text/html;charset="GB2312" AGTC——本元正阳
    尊敬的老师:
    您好!
    本元正阳公司年初推出的“腺相关病毒载体(AAV)服务套餐”优惠活动将于2005年4月20日截止。
     腺相关病毒(AAV2)服务超值套餐
    ——从腺相关病毒(AAV2)载体构建至生产5x1012v.g.纯病毒的一整套服务仅需15000元,可满足动物实验所需,并可以3000元的超低价格拥有等量(5x1012v.g.)携带报告基因的腺相关病毒(AAV2),在单价基础上为您节省了5000元的费用,大大降低了您的实验研究成本!
     腺相关病毒(AAV2/1)服务超值套餐
    ——从腺相关病毒(AAV2/1)载体构建至生产5x1012v.g.纯病毒的一整套服务仅需24000元,可满足动物实验所需,并可以3000元的超低价格拥有等量(5x1012v.g.)携带报告基因的腺相关病毒(AAV2/1),在单价基础上为您节省了5000元的费用,大大降低了您的实验研究成本!

    说明:AAV2/1——血清型1,AAV2的ITR,AAV1的外壳。
   :: 腺相关病毒(AAV2/1、AAV2)载体服务超值套餐 ::
 
服务项目
提供指标
所需时间
套餐价格(元)
单价(元)
备注
  A.实验方案设计
  根据您的研究项目需要,研发博士将协助您设计细胞及动物实验、确定给药途径及给药剂量等
 
免费
免费
实验方案设计仅供您参考。
  B.载体构建
a.质粒图谱(含电泳图)
b.构建流程
c.质粒酶切鉴定
d.PCR鉴定
e. 测序结果(含一个测序反应)
3周
2000
3000
如客户需要全基因测序,则每增一个测序反应加收200元。
  C.制备生产细胞株
  a. 载体细胞株的建立
  b. 高产的生产细胞株的筛选
10天
3000
4000
 
  D.病毒大规模生产
5x1012 v.g.
1个月
5000(AAV2)
14000(AAV2/1)
 
该病毒量可满足动物实验所需。
  E. 病毒分离纯化
  a. 细胞裂解
  b. 层析
  c. 过滤除菌
4天
3000
 
 
  F.质量检定
F1a. 基因组滴度测定(点杂交);
F1b. 纯度测定(SDS-PAGE);
F1c. 目的基因PCR测定;
F1d. 颗粒滴度测定
3天
2000
 
 
P. 对照腺相关病毒产品
P1. AAV2- eGFP(5x1012 v.g.)
现货
3000
6000
 
P2. AAV2-Luc(5x1012 v.g.)
P3. AAV2- LacZ(5x1012 v.g.)
 

本元正阳基因技术股份有限公司 版权所有
copyright ©2004-2006 All rights reserved.
--===============0384674122== Content-Type: text/plain; charset="us-ascii" MIME-Version: 1.0 Content-Transfer-Encoding: 7bit Content-Disposition: inline _______________________________________________ Bug-gnu-emacs mailing list Bug-gnu-emacs@gnu.org http://lists.gnu.org/mailman/listinfo/bug-gnu-emacs --===============0384674122==--