On Sep 14, 2021, at 11:29 AM, Augusto Stoffel > wrote: Hi Mark, thanks for testing. What happens if you start Emacs, then do 'M-x run-python', then 'M-x rename-buffer' to rename the Python shell as '*python-chain*', and then execute your notebook as usual? If this works, then the problem is in how ob-python initializes the shell. The Python shell needs a fraction of a second to start up, and if you send stuff to it before it's ready, it can be cause problems. Augusto, It runs as expected if I do that. As a summary of what I have tried that works is: - If I create the python buffer with this evaluation before running the org file/notebook. (python-shell-make-comint "python" "python-chain" nil) - If I don’t use the session header property to enable multiple src blocks to share the python session. - If I revert back to the python.el on August 27th. Possibly the older python.el was slow enough where I did not experience this problem. Thanks for introducing me to the trace-function. I will try that with the org babel functions to see if I can learn something more about the initialization process.